Hello, welcome to the official website of Weifang Huanuo Biological Technology



SYBR Green Ⅰ nucleic acid gel dye
Number of views:

SYBR Green Ⅰ nucleic acid gel dye

Retail price
Market price
Number of views:
Product serial number
  • 地址:山东省潍坊市寒亭区北海工业园珠江西二街
  • 手机:0536-8958326
  • 服务热线:400-168-2116
  • 邮箱:1004590214@qq.com
  • 1
    Product description
    Product Description
    SYBR Green I, a very sensitive dsDNA fluorescent dye, is commonly used in nucleic acid agarose gel and polyacrylamide gel electrophoresis.
    When using agarose electrophoresis to detect double-stranded DNA, SYBR Green Ⅰ is the most sensitive fluorescent dye. When it is bound to nucleic acid, it will produce strong fluorescence and high quantum yield. Because it can produce a strong signal after binding with nucleic acid, the background is very low, and the affinity for nucleic acid is high, so SYBR dye can be used under low concentration conditions.
    The lowest detection limit of SYBR GreenⅠ: 20pg DNA (254nm); 60pg DNA (300nm); In addition, SYBR GreenⅠ can also be used to detect oligonucleotides (1~2ng, 300nm UV transmission), which is 20 to 100 times more sensitive than EB . To
    When SYBR Green Ⅰ is used to detect DNA by electrophoresis, it can be pre-stained or stained after electrophoresis.
    After SYBR Green Ⅰ is used for agarose electrophoresis to detect DNA, the DNA can be directly transferred to the membrane for subsequent nucleic acid blot hybridization reactions. The combination of SYBR Green Ⅰ and DNA has no inhibitory effect on the activities of a variety of commonly used restriction enzymes, and can be directly digested or ligated.
    This product is SYBR Green I 10000× dye solution dissolved in DMSO.
    Specification: 100µL.
    Transportation and storage methods
    Ice pack transportation. Store the product at -20°C away from light.
    SYBR Green I is used in the same way as EB.
    Spot dyeing method:
    Take 1μL of the stock solution of this product and add 1mL of TE/TAE/TBE buffer, then add 1mL of 6×DNA Loading buffer and mix it as a working solution. During electrophoresis, mix 1~2μL of working solution and 5μL of electrophoresis sample and mix well. Load the sample directly after 5min.
    Paste dyeing method:
    Prepare 100 mL of agarose gel, heat it to melt, and add 10 μL of this product stock solution after the temperature drops to 50-60°C. After the gel has solidified, it can be electrophoresed, and it can be observed under ultraviolet irradiation after the electrophoresis is finished.
    1. SYBR Green Ⅰ should be stored in the dark, and the stock solution should be stored at -20°C; it is recommended to store in aliquots and freeze at 4°C for short-term storage.
    2. The gel staining method is highly sensitive, and the commercial DNA marker must be diluted 5-10 times before use.
    3. In the pre-staining method, the electrophoresis time should not exceed 2h, otherwise SYBR Green Ⅰ will be separated from the DNA and will produce diffuse bands.
    4. In the process of conventional alcohol precipitation of nucleic acids, SYBR Green I can be completely removed from double-stranded nucleic acids.
    5. SYBR Green I joined with double-stranded DNA shows green fluorescence under ultraviolet fluoroscopy. If the gel contains single-stranded DNA, the color is orange instead of green.
    6. SYBR Green I has a certain affinity for glass and non-polypropylene materials. It is recommended to use polypropylene containers in the process of dilution, storage, and dyeing.
    7. For your safety and health, please wear lab coats and disposable gloves for operation.





    Zhujiang West Second Street, Beihai Industrial Park, Hanting District, Weifang City, Shandong Province

    Username used for comment:

    Page Copyright 2021 Weifang Huanuo Biological Technology Co., Ltd.   鲁ICP备14017874号-2  Powered by 300.cn