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SYBR Green II Nucleic Acid Gel Stain
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SYBR Green II Nucleic Acid Gel Stain

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  • 地址:山东省潍坊市寒亭区北海工业园珠江西二街
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    Product description
    Product Description
    SYBR Green II is a very sensitive nucleic acid fluorescent dye that can stain RNA or single-stranded DNA.
    SYBR Green II dye does not specifically bind RNA or DNA single strands, but its binding efficiency to single strands is about twice that of double strands, and is widely used in RNA quality analysis. To
    When SYBR GreenII is used to detect RNA by electrophoresis, it can be pre-stained or stained after electrophoresis.
    After SYBR Green II is used for agarose electrophoresis to detect RNA, the RNA can be directly transferred to the membrane for subsequent nucleic acid blot hybridization reactions.
    Because it can produce a strong signal after binding with nucleic acid, the background is very low, and the affinity for nucleic acid is high, so SYBR Green II dye can be used under low concentration conditions.
    This product is SYBR Green II 10000× dye solution dissolved in DMSO.
    Specification: 100µL.
    Transportation and storage methods
    Ice pack transportation. Store the product at -20°C away from light.
    SYBR Green II is used in the same way as EB.
    Spot dyeing method:
    Take 1μL of the stock solution of this product and add 1mL of TE/TAE/TBE buffer, then add 1mL of 6×DNA Loading buffer and mix it as a working solution. During electrophoresis, mix 1~2μL of working solution and 5μL of electrophoresis sample and mix well. Load the sample directly after 5min.
    Paste dyeing method:
    Prepare 100 mL of agarose gel, heat it to melt, and add 10 μL of this product stock solution after the temperature drops to 50-60°C. After the gel has solidified, it can be electrophoresed, and it can be observed under ultraviolet irradiation after the electrophoresis is finished.
    1. SYBR Green II should be stored away from light, and the stock solution should be stored at -20°C; it is recommended to store in aliquots and freeze at 4°C for short-term storage.
    2. The gel staining method has high sensitivity, and the commercial RNA marker must be diluted 5-10 times before use.
    3. In the pre-staining method, the electrophoresis time should not exceed 2h, otherwise the SYBR Green II may separate from the RNA, resulting in diffuse bands.
    4. In the process of conventional alcohol precipitation of nucleic acids, SYBR Green II can be completely removed from single-stranded nucleic acids.
    5. SYBR Green II has a certain affinity for glass and non-polypropylene materials. It is recommended to use polypropylene containers in the process of dilution, storage, and dyeing.
    6. For your safety and health, please wear lab coats and disposable gloves for operation.





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