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Tth DNA polymerase

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    Product description
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    Product Description
    Tth DNA polymerase is a thermostable enzyme with a molecular weight of 94 kDa. It is recombinantly expressed in E. coli with the gene from Thermus thermophilus HB-8. The enzyme can replicate DNA at 74°C and has a half-life of 20 min at 95°C. Tth DNA polymerase can catalyze the polymerization of nucleotides along the 5'→3' direction in the presence of Mg2+ to form double-stranded DNA, or in the presence of Mn2+, use RNA as a template along the 5'→3' Nucleotide polymerization occurs in the direction. The enzyme also has 5'→3' exonuclease activity.
    Source: Recombinant Escherichia coli (Thermus thermophilus HB-8 gene).
    Uses: conventional PCR, RT-PCR.
    Specification: 5 U/µL.
    Transportation and storage methods
    Ice pack transportation. The product is stored at -20ºC.
    Activity definition
    Under the condition of 70ºC, the amount of enzyme required to catalyze the incorporation of 10nmol dNTP into TCA insoluble matter within 30 minutes is defined as 1 activity unit (U).
    Quality Inspection
    No exogenous nuclease and bacterial DNA contamination.
    purity
    SDS-PAGE:
    ≥97%.
    Reaction buffer
    10×Reverse transcription reaction buffer: 100mM Tris-HCl (pH 8.3, 25ºC), 900mM KCl;
    10×PCR reaction buffer: 500mM KCl, 100mM Tris-HCl (pH 9.0, 25ºC) and 1% Triton X-100.
    Precautions
    1. For your safety and health, please wear lab coats and disposable gloves for operation.
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