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Tth DNA polymerase
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Molecular Biological Enzymes
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Product description
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Product Description
Tth DNA polymerase is a thermostable enzyme with a molecular weight of 94 kDa. It is recombinantly expressed in E. coli with the gene from Thermus thermophilus HB-8. The enzyme can replicate DNA at 74°C and has a half-life of 20 min at 95°C. Tth DNA polymerase can catalyze the polymerization of nucleotides along the 5'→3' direction in the presence of Mg2+ to form double-stranded DNA, or in the presence of Mn2+, use RNA as a template along the 5'→3' Nucleotide polymerization occurs in the direction. The enzyme also has 5'→3' exonuclease activity.
Source: Recombinant Escherichia coli (Thermus thermophilus HB-8 gene).
Uses: conventional PCR, RT-PCR.
Specification: 5 U/µL.
Transportation and storage methods
Ice pack transportation. The product is stored at -20ºC.
Activity definition
Under the condition of 70ºC, the amount of enzyme required to catalyze the incorporation of 10nmol dNTP into TCA insoluble matter within 30 minutes is defined as 1 activity unit (U).
Quality Inspection
No exogenous nuclease and bacterial DNA contamination.
purity
SDS-PAGE: ≥97%.
Reaction buffer
10×Reverse transcription reaction buffer: 100mM Tris-HCl (pH 8.3, 25ºC), 900mM KCl;
10×PCR reaction buffer: 500mM KCl, 100mM Tris-HCl (pH 9.0, 25ºC) and 1% Triton X-100.
Precautions
1. For your safety and health, please wear lab coats and disposable gloves for operation.
Tth DNA polymerase is a thermostable enzyme with a molecular weight of 94 kDa. It is recombinantly expressed in E. coli with the gene from Thermus thermophilus HB-8. The enzyme can replicate DNA at 74°C and has a half-life of 20 min at 95°C. Tth DNA polymerase can catalyze the polymerization of nucleotides along the 5'→3' direction in the presence of Mg2+ to form double-stranded DNA, or in the presence of Mn2+, use RNA as a template along the 5'→3' Nucleotide polymerization occurs in the direction. The enzyme also has 5'→3' exonuclease activity.
Source: Recombinant Escherichia coli (Thermus thermophilus HB-8 gene).
Uses: conventional PCR, RT-PCR.
Specification: 5 U/µL.
Transportation and storage methods
Ice pack transportation. The product is stored at -20ºC.
Activity definition
Under the condition of 70ºC, the amount of enzyme required to catalyze the incorporation of 10nmol dNTP into TCA insoluble matter within 30 minutes is defined as 1 activity unit (U).
Quality Inspection
No exogenous nuclease and bacterial DNA contamination.
purity
SDS-PAGE: ≥97%.
Reaction buffer
10×Reverse transcription reaction buffer: 100mM Tris-HCl (pH 8.3, 25ºC), 900mM KCl;
10×PCR reaction buffer: 500mM KCl, 100mM Tris-HCl (pH 9.0, 25ºC) and 1% Triton X-100.
Precautions
1. For your safety and health, please wear lab coats and disposable gloves for operation.
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