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Inorganic pyrophosphatase
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Molecular Biological Enzymes
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Product Description
Inorganic pyrophosphatase can catalyze the hydrolysis of inorganic pyrophosphate to orthophosphate. Increase RNA yield and enhance DNA replication in reverse transcription reaction.
English name: Inorganic Pyrophosphatase.
CAS number: 9024-82-2.
Source: Recombinant Escherichia coli.
Specification: 0.5 U/µL.
Storage buffer
20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% (v/v) glycerol.
Transportation and storage methods
Ice pack transportation. Store the product at -20°C.
Activity definition
In a reaction system of 20 mM Tris-HCl (pH 7.5), 2 mM MgCl2, and 2 mM PPi, the amount of enzyme required to catalyze the formation of 1 μmol of phosphate from inorganic pyrophosphate at 25°C for 10 min is defined as 1 Units of activity (U).
Precautions
1. For your safety and health, please wear lab coats and disposable gloves for operation.
Inorganic pyrophosphatase can catalyze the hydrolysis of inorganic pyrophosphate to orthophosphate. Increase RNA yield and enhance DNA replication in reverse transcription reaction.
English name: Inorganic Pyrophosphatase.
CAS number: 9024-82-2.
Source: Recombinant Escherichia coli.
Specification: 0.5 U/µL.
Storage buffer
20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% (v/v) glycerol.
Transportation and storage methods
Ice pack transportation. Store the product at -20°C.
Activity definition
In a reaction system of 20 mM Tris-HCl (pH 7.5), 2 mM MgCl2, and 2 mM PPi, the amount of enzyme required to catalyze the formation of 1 μmol of phosphate from inorganic pyrophosphate at 25°C for 10 min is defined as 1 Units of activity (U).
Precautions
1. For your safety and health, please wear lab coats and disposable gloves for operation.
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