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Ribonuclease A
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Molecular Biological Enzymes
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Product description
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Product Description
Ribonuclease A (RNase A), a single-chain polypeptide with 4 disulfide bonds, has a molecular weight of approximately 13.7 kDa. As an endoribonuclease, it specifically degrades cytosine (C) or uracil (U) residues on single-stranded RNA. Specifically, cleavage recognizes the phosphodiester bond formed by the 5'-ribose on a certain nucleotide and the phosphate group on the adjacent 3'-ribose of the pyrimidine nucleotide, so that 2', 3' -Cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate (for example, pG-pG-pC-pA-pG is cleaved by RNase A to produce pG-pG-pCp and A-PG). RNase A has the highest activity in cutting single-stranded RNA, and is active under a variety of reaction conditions: at low salt concentration (0-100 mM NaCl), it can be used to cut single-stranded RNA, double-stranded RNA, and RNA-DNA hybrids. RNA strands, however, at high salt concentrations (≥0.3 M), RNase A only specifically cleaves single-stranded RNA.
RNase A is most commonly used in the removal of RNA during the preparation of plasmid DNA or genomic DNA. Therefore, the presence or absence of DNase enzyme activity during the preparation process is one of the pollution that needs attention. The traditional method of boiling in water bath can be used to inactivate DNase. . In addition, this product can also be used in molecular biology experiments such as RNase protection analysis and RNA sequence analysis.
Source: bovine pancreas.
Appearance: White or almost white freeze-dried powder.
Specification: 100 mg, 1 g.
Transportation and storage methods
Ice pack transportation. Store the product at -20°C.
Storage buffer
50 mM Tris-HCl (pH 7.4) and 50% (v/v) glycerol.
Content: ≥50U/mg.
Precautions
1. RNase A will be strongly adsorbed on glassware. It is recommended to put the solution in a plastic centrifuge tube.
2. For researchers who operate complete RNA experiments at the same time, they must be careful not to introduce RNase A to interfere with the accuracy of the test results.
3. For your safety and health, please wear lab coats and disposable gloves for operation.
Ribonuclease A (RNase A), a single-chain polypeptide with 4 disulfide bonds, has a molecular weight of approximately 13.7 kDa. As an endoribonuclease, it specifically degrades cytosine (C) or uracil (U) residues on single-stranded RNA. Specifically, cleavage recognizes the phosphodiester bond formed by the 5'-ribose on a certain nucleotide and the phosphate group on the adjacent 3'-ribose of the pyrimidine nucleotide, so that 2', 3' -Cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate (for example, pG-pG-pC-pA-pG is cleaved by RNase A to produce pG-pG-pCp and A-PG). RNase A has the highest activity in cutting single-stranded RNA, and is active under a variety of reaction conditions: at low salt concentration (0-100 mM NaCl), it can be used to cut single-stranded RNA, double-stranded RNA, and RNA-DNA hybrids. RNA strands, however, at high salt concentrations (≥0.3 M), RNase A only specifically cleaves single-stranded RNA.
RNase A is most commonly used in the removal of RNA during the preparation of plasmid DNA or genomic DNA. Therefore, the presence or absence of DNase enzyme activity during the preparation process is one of the pollution that needs attention. The traditional method of boiling in water bath can be used to inactivate DNase. . In addition, this product can also be used in molecular biology experiments such as RNase protection analysis and RNA sequence analysis.
Source: bovine pancreas.
Appearance: White or almost white freeze-dried powder.
Specification: 100 mg, 1 g.
Transportation and storage methods
Ice pack transportation. Store the product at -20°C.
Storage buffer
50 mM Tris-HCl (pH 7.4) and 50% (v/v) glycerol.
Content: ≥50U/mg.
Precautions
1. RNase A will be strongly adsorbed on glassware. It is recommended to put the solution in a plastic centrifuge tube.
2. For researchers who operate complete RNA experiments at the same time, they must be careful not to introduce RNase A to interfere with the accuracy of the test results.
3. For your safety and health, please wear lab coats and disposable gloves for operation.
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