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Reverse transcriptase
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Category
Molecular Biological Enzymes
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Product description
有效活菌数:
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Product Description
M-MLV reverse transcriptase has RNA and DNA-dependent polymerase activity and RNase H activity. The RNase H activity of this enzyme is much lower than that of avian myeloblastoma reverse transcriptase (AMV).
Efficiently prepare full-length first-strand cDNA, up to 13 kb in length.
The best activity temperature is 42°C. It is still active at temperatures up to 50°C.
Source: Recombinant Escherichia coli (reverse transcriptase gene of mouse leukemia virus).
Specification: 200 U/µL.
Application
1. First-strand cDNA synthesis for RT-PCR and real-time RT-PCR;
2. Synthesize cDNA for cloning and expression research;
3. Preparation of labeled cDNA probes for chip research;
4. DNA marking;
5. Analyze RNA by primer extension method.
Transportation and storage methods
Ice pack transportation. Store the product at -20°C.
Activity definition
Under the condition of 37°C, the amount of enzyme required to synthesize 1 nmol dTMP into the polymerized nucleotide fragment within 10 minutes is defined as 1 activity unit (U).
Storage buffer
50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, 50% (v/v) glycerol.
5X Reaction Buffer
250 mM Tris-HCl (pH8.3), 250 mM KCl, 20 mM MgCl2, 50 mM DTT.
Inhibitor
Metal chelating agents, inorganic phosphates, pyrophosphate and polyamines.
Inactivation
Heat at 70°C for 10 min.
Precautions
1. For your safety and health, please wear lab coats and disposable gloves for operation.
M-MLV reverse transcriptase has RNA and DNA-dependent polymerase activity and RNase H activity. The RNase H activity of this enzyme is much lower than that of avian myeloblastoma reverse transcriptase (AMV).
Efficiently prepare full-length first-strand cDNA, up to 13 kb in length.
The best activity temperature is 42°C. It is still active at temperatures up to 50°C.
Source: Recombinant Escherichia coli (reverse transcriptase gene of mouse leukemia virus).
Specification: 200 U/µL.
Application
1. First-strand cDNA synthesis for RT-PCR and real-time RT-PCR;
2. Synthesize cDNA for cloning and expression research;
3. Preparation of labeled cDNA probes for chip research;
4. DNA marking;
5. Analyze RNA by primer extension method.
Transportation and storage methods
Ice pack transportation. Store the product at -20°C.
Activity definition
Under the condition of 37°C, the amount of enzyme required to synthesize 1 nmol dTMP into the polymerized nucleotide fragment within 10 minutes is defined as 1 activity unit (U).
Storage buffer
50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, 50% (v/v) glycerol.
5X Reaction Buffer
250 mM Tris-HCl (pH8.3), 250 mM KCl, 20 mM MgCl2, 50 mM DTT.
Inhibitor
Metal chelating agents, inorganic phosphates, pyrophosphate and polyamines.
Inactivation
Heat at 70°C for 10 min.
Precautions
1. For your safety and health, please wear lab coats and disposable gloves for operation.
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