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T4 polynucleotide kinase
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Molecular Biological Enzymes
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Product Description
T4 polynucleotide kinase can catalyze the transfer of the γ-position phosphate group of ATP to the 5′-hydroxyl end of the oligonucleotide chain (double-stranded or single-stranded DNA or RNA) and the 3′-monophosphate nucleoside.
T4 polynucleotide kinase also has 3′ phosphatase activity, which removes the 3′-phosphate group from the 3′ phosphate end of the oligonucleotide, deoxy 3′-monophosphate nucleoside and deoxy 3′-diphosphate nucleoside Hydrolyzed off.
Source: Recombinant Escherichia coli (T4 polynucleotide kinase gene).
Application
1. Phosphorylation of the 5'end of DNA or RNA for ligation reaction;
2. End labeling of DNA or RNA for use as probes and DNA sequencing;
3. Use T4 polynucleotide kinase to remove the 3'phosphate group.
Specification: 10 U/µL.
Storage buffer
10 mM Tris-HCl (pH 7.4), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1 µM ATP, 50% (v/v) glycerol.
5×T4 PNK Buffer
700 mM Tris-HCl (pH 7.6), 100 mM MgCl2, 50 mM DTT.
Transportation and storage methods
Ice pack transportation. Store the product at -20°C.
Activity definition
At 37°C, the amount of enzyme required to catalyze the incorporation of 1 nmol of acid-insoluble [32P] in 30 minutes is defined as 1 activity unit (U).
Inactivation
Heat at 65°C for 20 min.
Quality Inspection
No Endonuclease, Exonuclease, Rnase pollution.
Purity
SDS-PAGE: ≥99%.
Precautions
1. For your safety and health, please wear lab coats and disposable gloves for operation.
T4 polynucleotide kinase can catalyze the transfer of the γ-position phosphate group of ATP to the 5′-hydroxyl end of the oligonucleotide chain (double-stranded or single-stranded DNA or RNA) and the 3′-monophosphate nucleoside.
T4 polynucleotide kinase also has 3′ phosphatase activity, which removes the 3′-phosphate group from the 3′ phosphate end of the oligonucleotide, deoxy 3′-monophosphate nucleoside and deoxy 3′-diphosphate nucleoside Hydrolyzed off.
Source: Recombinant Escherichia coli (T4 polynucleotide kinase gene).
Application
1. Phosphorylation of the 5'end of DNA or RNA for ligation reaction;
2. End labeling of DNA or RNA for use as probes and DNA sequencing;
3. Use T4 polynucleotide kinase to remove the 3'phosphate group.
Specification: 10 U/µL.
Storage buffer
10 mM Tris-HCl (pH 7.4), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1 µM ATP, 50% (v/v) glycerol.
5×T4 PNK Buffer
700 mM Tris-HCl (pH 7.6), 100 mM MgCl2, 50 mM DTT.
Transportation and storage methods
Ice pack transportation. Store the product at -20°C.
Activity definition
At 37°C, the amount of enzyme required to catalyze the incorporation of 1 nmol of acid-insoluble [32P] in 30 minutes is defined as 1 activity unit (U).
Inactivation
Heat at 65°C for 20 min.
Quality Inspection
No Endonuclease, Exonuclease, Rnase pollution.
Purity
SDS-PAGE: ≥99%.
Precautions
1. For your safety and health, please wear lab coats and disposable gloves for operation.
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