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Uracil Glycosylase

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  • 地址:山东省潍坊市寒亭区北海工业园珠江西二街
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    Product description
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    Product Description
    Uracil DNA glycosylase (UDG) is derived from E. coli recombinant clone expression. With a molecular weight of 25kDa, it can catalyze the release of free uracil from single-stranded and double-stranded DNA containing uracil. It has no activity on RNA.
    Source: Recombinant Escherichia coli.
    Use
    PCR cross contamination control;
    Glycokinase-mediated single nucleotide polymorphism detection (GMPD);
    Site-specific mutation;
    Probes for the study of protein-DNA related effects;
    SNP genotyping;
    PCR product cloning;
    Single-stranded overhangs are generated in PCR products and cDNA.
    Specification: 1U/µL.
    Transportation and storage methods
    Ice pack transportation. Store the product at -20°C.
    Storage buffer
    30 mM HEPES-KOH (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.05% (v/v) Tween-20, 50% (v/v) glycerol.
    Activity definition
    At 37°C, the amount of enzyme required to release 1nmol of uracil from a DNA template containing uracil within 60 minutes is defined as 1 activity unit (U).
    Inactivation
    Heat at 95°C for 10 minutes. When the temperature is lower than 55°C, the enzyme activity is partially reduced. Therefore, after PCR amplification, the PCR product can be directly loaded onto the gel electrophoresis after ice bath.
    Quality Inspection
    No endo-or exo-deoxyribonuclease, ribonuclease, phosphatase contamination.
    Precautions
    1. For your safety and health, please wear lab coats and disposable gloves for operation.
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